دانلود رایگان مقاله ارزیابی ضد باکتری و آنتی بیوفیلم عصاره میوه خیار تلخ

abstract

Silver nanoparticles were synthesized using the fruit extracts of Momordica charantia (Mc-AgNPs). The structural characterization of Mc-AgNPs was performed by UV–vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). UV–vis recorded the absorbance spectra at 460 nm. XRD shows the crystalline nature of silver nanoparticles with various Bragg's reflection peaks at 111, 200, 220 and 311. FTIR spectra of the synthesized Mc-AgNPs showed strong bands at 1382, 1203, 1151, 1102, 1013 and 654 cm
1 which corresponds to O–H, C–H, C–C, C–OH and C–N groups. TEM showed the spherical shape of Mc-AgNPs with particle size between 16 nm. The antibacterial activity of Mc-AgNPs was tested against Gram-positive and Gram-negative bacteria. McAgNPs showed greater inhibition of Enterococcus faecalis at 100 mg ml
1 compared to Aeromonas hydrophila. The biofilm inhibition of Mc-AgNPs was also higher against E. faecalis compared to A. hydrophila

5. Conclusion

Silver nanoparticle was easily synthesized using the fruit extract of M. charantia (Mc-Ag NPs). The synthesized Mc-Ag NPs showed enhanced antibacterial activity against Gram positive E. faecalis than Gram negative A. hydrophila. The antibiofilm activity was also greater against E. faecalis than A. hydrophila. Mc-Ag NPs reduced the biofilm growth of bacteria by decreasing their hydrophobicity index. This study concludes that Mc-Ag NPs could be effectively used in the control of pathogenic bacteria.