دانلود رایگان مقاله بیان ژن های سرکوبگر تومور مربوط به چرخه سلولی در بیماران مبتلا به سرطان آندومتر

Abstract

Purpose Endometrial cancer is the most common gynecological malignancy in developed countries. The role of tumor suppressor genes (TSG) in endometrioid endometrial adenocarcinoma (EEC) has an important impact on patient survival prognosis. Thus, it is important to identify TSG transcripts that differentiate endometrial adenocarcinoma into various pathomorphological grades. The aim of this study was to analyze the expression profile of tumor suppressor genes related to the cell cycle in patients with endometrial adenocarcinoma across histological differentiation and to identify transcripts which differentiate endometrium into various pathomorphological grades. Material and methods Gene expression analysis was completed for 19 endometrial endometrioid adenocarcinomas and 5 normal specimens (obtained from women with diagnosed uterine fibroids, benign ovarian tumors and a prolapsed uterus with histopathologically confirmed endometrium in the proliferative phase) using Affymetrix HG-U133A oligonucleotide microarrays. The statistical analysis was performed using the GeneSpring13.0 software and PANTHER classification system. Results Significant changes in gene expression were observed across histological differentiation. The WT-1, CYR 61, TSPYL5 genes were statistically and biologically significant in all cancer grades, and were considered to be primary for the G1 grade in endometrial cancer. The G2 cancer specific genes were BCL2L2 and HNRNPA0, whereas in G3 there was only BAK. Conclusion In conclusion, the WT-1, CYR61 and TSPYL5 gene expressions are potentially correlated with patient survival in all endometrial cancer grades. The TSGs identified are considered to be important in EEC pathogenesis and further research is needed to confirm this.

5. Conclusions

We can conclude that the WT-1, CYR61 and TSPYL5 gene expressions are important in endometrial cancerogenesis and patient prognosis. Their regulation has an influence on survival and can be of importance in therapy. The TSGs related to the cell cycle in G2 and G3 endometrial adenocarcinoma are mostly correlated to apoptosis. Changes in the expression of BAK and BCL2L2 suggest pro-survival signaling and inhibition of apoptosis leading to uncontrolled cancer cell proliferation. However, further study on the topic is needed. The identified TSGs can be considered as potential prognostic markers of endometrial adenocarcinoma in Polish women