- مبلغ: ۸۶,۰۰۰ تومان
- مبلغ: ۹۱,۰۰۰ تومان
Hairy Root Culture is the new route for large scale secondary metabolite production because of their fast and plagiotropic growth, genetic and biochemical stability. Axenic explants of four medicinally important plants Aegle marmelos, Boerhavia dif usa, Datura innoxia and Solanum xanthocarpum were used for induction of Hairy Root Cultures using the Agrobacterium rhizogenes strain MTCC 532, by stabbing, co-culturing and cut end infection methods to establish cultures of transformed roots. We were able to induce Hairy Roots in three plants except Aegle marmelos by stabbing method with high transformation frequency (60-75%). In Solanum xanthocarpum transformation frequency was highest (75%) followed by Datura innoxia (62.8%) and Boerhavia dif usa (60%). Copious growth of Solanum xanthocarpum Hairy Roots was observed in ½ MS liquid medium without hormones with growth index of 45 with doubling time of 7 days. Further this study creates a paradigm for future scale up of hairy roots in bioreactors for large scale production of secondary metabolites & its increasing use as model system in metabolic engineering.