دانلود رایگان مقاله انگلیسی یک روش حساس و سریع برای تشخیص فرمالدئید در بافتهای مغزی - هینداوی 2017

The existing methods for detecting formaldehyde (FA) in brain samples are expensive and require sophisticated experimental procedures. Here, we established a highly sensitive and selective spectrophotometric method, which is based on a reaction in which FA reacts with colorless reagent 4-amino-3-penten-2-one (Fluoral-P) to produce a yellow compound, 3,5-diacetyl-1,4- dihydrolutidine (DDL), which can be detected by a spectrophotometer at 420 nm at room temperature. The sensitive response time point was found to be at the first hour, and the optimal pH of derivative reaction was pH 6.0. The limit of detection (LOD) and the limits of quantization (LOQ) for detecting FA were 0.5 μM and 2.5 μM, respectively. Using this method, an abnormally high level of FA was detected in both the brains of FA-injected mice and autopsy hippocampus tissues from patients with Alzheimer’s disease. This finding suggests that the modified Fluoral-P method is effective for measuring levels of FA in the brains.

5. Conclusion Using this modified Fluoral-P method, an abnormally high level of FA was detected in brain samples of FA-injected mice (Supplementary Figure 3). Taken together, the present spectrophotometric method using Fluoral-P as a derivative reagent for measurement of FA in brain samples constitutes a simple, sensitive, and practicable alternative to well-established methods for determining brain FA and assessing neurotoxicity of excess FA.