ترجمه مقاله نقش ضروری ارتباطات 6G با چشم انداز صنعت 4.0
- مبلغ: ۸۶,۰۰۰ تومان
ترجمه مقاله پایداری توسعه شهری، تعدیل ساختار صنعتی و کارایی کاربری زمین
- مبلغ: ۹۱,۰۰۰ تومان
abstract
Exopolysaccharide (EPS) productivity of the slow-growing Ganoderma lucidum BCCM 31549 mushroom was enhanced in repeated batch fermentation (RBF) process by reducing the fermentation time. The results showed that by using 2.5-L STR unbaffled bioreactor, the fermentation time could be reduced from 10 days per batch to 5 days, due to lag-phase elimination by adopting a RBF strategy. RBF approach was also shown to achieve a semi-continuous mode of production by eliminating the inoculation time between each cycle. The fermentation period was shortened from 50 to 25 days in five consecutive cycles of RBF, with the productivity of EPS increased from 0.81 g/L day1 to 1.32 g/L day1 by using N-limiting medium. The presence of clamp connections in RBF ensures the reproducibility of this strategy. The proposed strategy proved to provide easy operation and thus can be adapted for EPS production
4. Conclusions
The optimized repeated-batch strategy shortened the fermentation period and enhanced the EPS productivity significantly, which generated strong potential for the large-scale EPS production. The presence of clamp connections in RBF strategy ensures the reproducibility of the fungus growth in the liquid environment for both in the shake flask and bioreactor.