A 4-hour-old mixed-breed black heifer calf produced from in vitro fertilization and born via Caesarian section 6 days prior to the anticipated due date was euthanized because of multiple obvious congenital anomalies and an inability to stand. The number of congenital anomalies was remarkable and included anal and vulvar atresia, hypoplastic clitoral tissue, cervical block vertebrae, anotia, brachygnathia inferior, secondary cleft palate, calvarial doming with hydrocephalus and stenosis of the Sylvian aqueduct, high ventricular septal defect, rectovaginal fistula, and dermal hamartomas. Few previous reports of congenital anomalies in calves have documented the number and the severity of those seen in this calf. Because development of tissues and organs is interdependent, it is not uncommon for more than 1 anomaly to appear in an animal. In this calf, several rarely reported anomalies, such as anotia, vulvar atresia, and dermal hamartomas, were evident; these anomalies have not been previously documented in cattle.