- مبلغ: ۸۶,۰۰۰ تومان
- مبلغ: ۹۱,۰۰۰ تومان
Urtica dioica (UD) with a long history of usage in folkloric and science based herbal medicine is dioeciously and has therapeutic applications. This study was aimed to assess the effect of nettle root extract UD on the testes of adult rats. Eighty adult male rats were randomly divided into 8 groups. Three groups received 300, 600 and 1000 mg/kg/day of hydro-alcoholic extract of nettle root intraperitoneally for 3 days for determining lethal dose. One group, as control and another as sham and three others received 75, 150, 300 mg/kg/day for 20 days. The rats were then sacrificed and serum levels of FSH, LH and testosterone were determined by radioimmunoassay. The testes removed from the body and then stained tissue samples, changes of testicular tissue were studied and data collected were analyzed. There was a reduction in the mean body weight in the experiment group 3 (300 mg/kg/day) at the end of the treatment. The mean number of spermatogonia per tubular tubules, Leydig cells/mm2 in diameter tubules (µm) in the experiment group3 compared to experiment group1 (75 mg/kg/day) decreased significantly (P<0.05). However, the average diameter of the tubules of the experimental group 2 (150 mg/kg/day) and group 3 (300mg/kg/day) compared to control and sham groups decreased significantly (P<0.05). This study showed that administration of nettle root extract, at a dose of 75 mg/kg has had a positive effect on spermatogenesis and with 300 mg/kg dose has had a negative effect on spermatogenesis.