دانلود رایگان مقاله سرامید سیتوتوکسیک Heteroxenia ghardaqensis و اثر حفاظتی عصاره کلروفرم

Abstract

A new ceramide 2S,3R-4E,8E-2-(hexadecanoylamino)-docosa-4,8-diene-1,3-diol (1), along with two known ceramides, namely, 2S,3R-4E,8E-2-(octadecanoylamino)-octadeca -4,8-diene-1,3-diol (2) and 2S,3R-4E-2-(octadecanoylamino)-octadec-4-ene-1-ol (3) were isolated from the chloroform extract of the Egyptian Red Sea soft coral Heteroxenia ghardaqensis. Additionally, cholesterol, cholesterol-3b-acetate, 7b-hydroxy cholesterol, cosanyl octadecanoate, and hexadecanyl octadecanoate were also isolated. The isolated compounds were identified by spectroscopic methods involving 1D, 2D-NMR and ESI-MS. The ceramides (1) and (2) showed moderate cytotoxic activity as growth inhibitors of human hept-G2 cancer cell lines. The protective action of the chloroform extract on cadmium-induced adrenal toxicity was observed by the decrease of the hydropic degeneration in the cortex and normal medulla.

4. Conclusion

A new ceramide, 2S,3R-4E,8E-2-(hexadecanoylamino)-docosa- 4,8-diene-1,3-diol (1), along with seven known compounds was isolated from the CHCl3 extract of soft coral H. ghardaqensis. The two ceramides (1) and (2) showed moderate cytotoxic activity as growth inhibitors of human hep-G2 cancer cell lines. The protective action of the CHCl3 extract on cadmium-induced adrenal toxicity was observed by the decrease of the hydropic degeneration in the cortex and normal medulla. The protective role of H. ghardaqensis could be useful in the adrenal gland protection against cadmium.