- مبلغ: ۸۶,۰۰۰ تومان
- مبلغ: ۹۱,۰۰۰ تومان
Sucrose is one of the most promising carbon sources for industrial fermentation. We expressed synthetic modules expressing genes of the PEP-PTS and non-PTS pathways in Escherichia coli K12 for comparison. We selected PEP-PTS pathway genes of Lactobacillus plantarum and Staphylococcus xylosus and non-PTS pathway genes of sucrose-utilizing (Scr+ ) E. coli EC3132. Switchable Scr+ modules expressing E. coli EC3132 non-PTS genes conferred better sucrose-utilizing ability on Scr E. coli K12 than E. coli EC3132. Scr+ modules expressing S. xylosus PEP-PTS genes conferred a sucrose-utilizing ability on E. coli K12. Among L. plantarum PEP-PTS genes, SacALP and SacKLP were functional in E. coli K12. CscAEC–CscBEC– CscKEC (non-PEP-PTS module) or ScrASX–SacALP–SacKLP (PEP-PTS module) was introduced to a diapolycopene-producing E. coli strain. In both Scr+ E. coli K12, the sucrose-utilizing ability of the modules was not affected by diapolycopene formation, indicating that the modular Scr+ systems could be employed for developing sustainable bioprocesses using sucrose.