دانلود رایگان مقاله محیط زیست ناشی از رفتار خود تجمعی κ-کاراگینان / لیزوزیم پیچیده

Abstract

Self-aggregation behaviors of κ-carrageenan/lysozyme (CRG/Ly) complex inducing by intrinsic and extrinsic factors were investigated form macro and micro aspects, including the ratio, pH, temperature and salt. High Ly content promote aggregation behavior of CRG/Ly complex, while CRG played a suppressive role. Except for CRY5, the complex displayed nano-size distribution. Both CRG addition and the self-aggregation behavior could enhance the thermal stability of Ly. Low ζ-potential induced by pH made the CRG/Ly complex easy to self-aggregate into structure with large size due to the gradual association of complex to form lager interpolymeric complexes. The tunable process means to control the dissociation and association behavior between interpolymeric complexes and soluble complex by pH. All systems shared high NaCl tolerance and presented stable transmittance. Heat-induced self-aggregation exhibited a temperature and polymers ratios dependant manner. The mechanisms of environment induced self-aggregation into interpolymeric complexes reflected the re-balanced physical interaction when they suffered from any treatment. Controlling and regulating self-aggregation behavior of protein/polysaccharide system could enrich the soft structures from food respective and also provide practical theory for food process.

4. Conclusions

Environment induced self-aggregation behaviors of CRG/Ly complex were investigated by transmittance, size, ζ-potential and micro-structure analysis. The self-aggregation behavior was significantly affected by the CRG/Ly ratios. High Ly content could accelerate the aggregation behavior of CRG/Ly complex, while CRG content played a contrary role. CRG/Ly complex system displayed nano-size distribution except for CRY5. Thermal stability of Ly was improved by CRG addition and the self-aggregation behavior. The dissociation and association behavior between interpolymeric complexes into soluble complex could be regulated by pH. All CRG/ Ly system shared high NaCl tolerant behavior and presented stable transmittance with slight increase in wide range of NaCl concentrations. Heat-induced self-aggregation exhibited a temperature and polymers ratios dependant manner. Temperature elevation promoted the formation of interpolymeric complexes. Environment induced self-aggregation into interpolymeric complexes also re- flected the re-balanced physical interaction between the polymers and their complex when their system suffered any environmental changes. The self-aggregation behavior was the gradual association of CRG/Ly complex to form interpolymeric complexes with a large size. Regulating self-aggregation behavior of CRG/Ly system could enrich the nano/micro structures from soft material respective and also provide practical theory for food process.