دانلود رایگان مقاله انگلیسی تغذیه خونی توسط پشه ماده Lutzomyia longipalpis: افزایش با تخمگذاری و اثرات آن بر هضم - هینداوی 2018

Lutzomyia longipalpis is the main vector of visceral leishmaniasis (VL) in America. Physiological and molecular mechanisms of Leishmania infection in sand fies have been studied during the frst gonotrophic cycle.Tere are few studies about these interactions during the second gonotrophic cycle mainly because of the difculties maintaining sand fies through sequential feeds. Here we standardized conditions to perform the second blood feed efciently, and our results show that oviposition is an essential factor for the success of multiple feeds. We evaluated the impact of the second blood meal on longevity, protein digestion, trypsin activity, and Leishmania mexicana development within L. longipalpis gut. Mortality of blood-fed females increases afer second blood meal as compared to sugar-fed females. Trypsin activity was lower during the second gonotrophic cycle. However, no diference in protein intake was observed between blood meals. Tere was no diference in the population size of Leishmania in the gut afer both blood meals. In this work, we presented an optimized protocol for obtaining sufcient numbers of sand fy females fed on a second blood meal, and we described some physiological and parasitological aspects of the second gonotrophic cycle which might infuence the vectorial competence of sand fies.

5. Conclusions

We have standardized a method to increase the number of engorged females afer a second blood feeding, which will facilitate future work in this research area. Additionally, we have shown that midgut trypsin activity changes according to the gonadotrophic cycle, which might ultimately infuence the vectorial competence of sand fies and transmission of disease.