دانلود رایگان مقاله نانودوت کربن فلورسنت برای تصویربرداری سلول های سرطانی

Abstract

Carbon quantum dots (CQDs or C-dots, ≤10 nm in size) are tiny carbon nanoparticles being envisaged in biosensing, bio-imaging and biomolecular/drug delivery. In the present investigation, green fluorescent carbon quantum/nano dots (GCQDs, ∼3 nm in size) were synthesized through facile chemical slicing method. Further, folic acid (FA) functionalized GCQDs (GCQDs-FA) were obtained to enhance their targeting ability. FA is known to positively influence the binding potential and penetration into the cancer cells because of high abundance of folate receptors (FR) on various cancer cell membranes. We report high biocompatibility, photoluminescence stability and excellent in vitro cancer cell cytoplasm and nucleus targeting performance of GCQDs-FA on MCF-7 breast cancer cells.

4. Conclusions

Green fluorescence of GCQDs-FA in MCF-7 breast cancer cells observed under fluorescence microscopy imaging is in accordance with the green fluorescence of acidic aqueous suspension of GCQDs obtained in PL spectra. We have demonstrated a one pot large scale synthesis for producing green fluorescent carbon quantum dots (GCQDs) of ∼3 nm in size. The synthesis process ensured (1) one pot process (>50% conversion efficiency), (2) ambient reaction conditions, (3) excellent green photoluminescence and prolonged stability, (4) good water solubility and (5) superior biocompatibility. Due to low toxicity and good fluorescent nature, the GCQDs-FA was successfully used for targeted in vitro cell imaging (MCF-7 breast cancer cells). Further, excellent cancer cell cytoplasm and nucleus targeting performance of folic acid functionalized GCQDs (GCQDs-FA) has been demonstrated. A dual targeting ability (cytoplasm and nucleus) by using GCQDs-FA as a single platform has been observed.