دانلود رایگان مقاله انگلیسی لیموترش تونس: خواص فیتوشیمیایی و فیزیکوشیمیایی و فعالیت های بیولوژیکی - هینداوی 2018

Natural plant extracts contain a variety of phenolic compounds which are assigned various biological activities. Our work aims to make a quantitative and qualitative characterization of the Zest (ZL) and the Flesh (FL) of lemon (Citrus limon), to valorize the pharmacological uses of lemon, by evaluating in vitro activities (DPPH, free radical scavenging and reducing power). The antibacterial, antifungal, and antiproliferative activities were sought in the ability of Citrus limon extracts to protect DNA and protein. We found that the ZL contains high amounts of phenolics responsible for the important antioxidant properties of the extract. However, the FL is richer in flavonoids than the ZL. The FL extract was also found to be more effective than the ZL in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals. We also concluded that the FL extract exhibited potent antibacterial activity unlike ZL. Analysis by LC/MS-MS identified 6 compounds (Caffeoyl N-Tryptophan, Hydroxycinnamoyl-Oglucoside acid, Vicenin 2, Eriocitrin, Kaempferol-3-O- rutinoside, and Quercetin-3-rutinoside). These preliminary results showed that Citrus limon has antibacterial and antioxidant activity in vitro. It would be interesting to conduct further studies to evaluate the in vivo potential in an animal model.

4. Conclusion

In conclusion, the results of the present study indicate that the extracts from Citrus limon exhibit powerful antioxidant properties, expressed by its capacity to scavenge DPPH radicals and to reduce power, and the extracts reduce H2O2- induced DNA via its antioxidant activities. These antioxidant activities and inhibitory effects of the extracts on DNA and cell damage may further prove that Citrus limon is useful as a medicinal plant for cancer chemoprevention.

The results obtained show that Citrus limon extracts contain high enough levels of phenolic and flavonoid compounds. This is correlated with a remarkable antioxidant activity towards the reduction of iron, and a relatively high power against scavenging free radicals. So, Citrus limon extracts could be a promising antioxidant source for the prevention and/or treatment of oxidative stress-related diseases or as food additives.