دانلود رایگان مقاله انگلیسی خواص ضد میکروبی فیلم نانوکامپوزیتی نقره ای کیتوزان علیه باکتری و قارچ پاتوژن ماهی - اشپرینگر 2017

Abstract

Development of nanostructured films using natural polymers and metals has become a considerable interest in various biomedical applications. Objective of the present study was to develop silver nano particles (AgNPs) embedded chitosan films with antimicrobial properties. Based on the Ag content, two types of chitosan silver nano films, named as CAgNfs-12 (12 mM) and CAgNfs-52 (52 mM) were prepared and characterized. Field emission scanning electron microscope (FE-SEM) images of two CAgNfs showed the circular AgNPs, which were uniformly embedded and distributed in the matrix of chitosan films. Antimicrobial experiment results clearly indicated that CAgNfs can inhibit the growth of fish pathogenic bacteria Vibrio (Allivibrio) salmonicida, V. tapetis, Edwardsiella tarda and fungi Fusarium oxysporum. Moreover, CAgNfs significantly reduced the experimentally exposed V. salmonicida levels in artificial seawater, suggesting that these CAgNfs could be used to develop antimicrobial filters/membranes for water purifying units to eliminate the pathogenic microbes.

Conclusions

In conclusions, AgNPs were successfully introduced into chitosan using a green reduction method in order to develop biologically active CAgNfs. Incorporation of Ag was confirmed by the SPR peak at 415 nm for both CAgNfs indicating the formation of AgNPs. Results from disc diffusion and turbidimetric assays clearly indicated that both CAgNfs can inhibit the growth of pathogenic bacteria (V. salmonicida, V. tapetis and E. tarda) as well as fungi F. oxysporum. These CAgNfs could be developed or modified into antimicrobial filters/membranes for water filtering units to eliminate the pathogenic bacteria and fungi in future.