Abstract
In this study we have investigated micropropagation and in vitro flowering for a medicinally important plant Pentanema indicum. Maximum callus proliferation was obtained on MS medium supplemented with 2.0 mg l۱ BAP and 1.0 mg l۱ IBA. The best shoot regeneration (191.0) was achieved in five weeks when callus was cultured on MS medium amended with 4.0 mg l۱ BAP and 1.0 mg l۱ IAA. Direct multiple shoot initiation was also obtained from shoot tip and nodal explants in the presence of BAP and IAA. Addition of adenine sulfate (1.0 mg l۱ ) to the regeneration medium increased the shoot multiplication. Regenerated shoots rooted best on MS medium containing 2.0 mg l۱ IBA. Multiple shoots regenerated from callus, shoot tip and nodal explants flowered (90%) in vitro on a MS medium fortified with 2.0 mg l۱ IBA. Plantlets were successfully acclimatized in a soil condition and the survival rate was 96%.
