دانلود رایگان مقاله بروز اینترلوکین 23p19 در بیماران مبتلا به کولیت اولسروز

Summary

Background: The purpose of this study was to determine whether the mucosal expression of interleukin (IL)-23p19 has a role in the pathogenesis of ulcerative colitis, and to determine its relation to disease severity. Methods: This study was performed on 50 patients with ulcerative colitis and 10 normal individuals as the controls. They were divided into Group I (27 patients with mild to moderate disease), Group II (23 patients with severe disease), and Group III (10 normal individuals). All patients and the controls were subjected to histopathological study, IL-23p19 immunohistochemical staining, IL-23R expression by flow cytometry, and serum IL-23 by enzyme-linked immunoassay. Results: There was a significant increase in IL-23p19 gene expression and IL-23R level in patients with ulcerative colitis, compared to the controls. A significant positive correlation was detected between increased expression of the IL-23p19 gene, IL-23R, high serum IL-23, and the severity of the disease. Conclusion: Increased expression of the IL-23p19 gene has a role in the pathogenesis of ulcerative colitis. Targeted therapy directed against IL-23p19 may be effective in its treatment. Increased expression of the IL-23p19 gene and IL-23R with high serum IL-23 is correlated positively with disease severity.

Discussion

Patients with UC are at increased risk of inflammation. Interleukin-23 is a newly identified cytokine with increased expression in inflamed biopsies of colon mucosa in patients with CD; however, there is inconsistent evidence on its role in UC [14]. Interleukin-23 is a heterodimeric cytokine that shows similar function as IL-12 in promoting cellular immunity and enhancing lymphocyte proliferation [15]. This study showed significant increased expression of the IL-23p 19 gene in patients with UC, compared to the controls. This increase was significantly higher in patients with severe UC disease, compared to patients with mild to moderate disease. This was similar to the results reported by Schmidt et al [16], Zhanju et al [13], and Kobayashi et al [17]. We observed that, based on immunohistochemistry, IL-23p19-positive cells were primarily macrophages. This was in agreement with the findings of Zhanju et al [13].