دانلود رایگان مقاله بیوسنتز 4-استیل آنتراکوئینول B در قارچ آترود یا سینا مومیا

Abstract

The biosynthesis pathway for production of 4-acetylantroquinonol B (4-AAQB) by Antrodia cinnamomea was investigated by adding various precursors to the culture medium. Adding 4-hydroxybenzoic acid (4-HBA) significantly increased the production of 4-AAQB. Since 4-HBA is an intermediate of the shikimate pathway and 4-AAQB and coenzyme Q (CoQ) are similar in structure, we suspected that the pathway for producing 4-AAQB was closely related to the biosynthesis of CoQ. Since the isoprenoid chain of CoQ is synthesized via the mevalonate pathway, we added oleic acid to the culture medium and confirmed that the addition significantly increased the production of 4-AAQB. Furthermore, adding coenzyme Q0 into the fermentation broth was found to be the most effective way to increase the production of 4-AAQB. We suspect that coenzyme Q0 forms CoQ, after which CoQ is converted to 4-AAQB via unknown steps. The increase in 4-AAQB production due to the addition of CoQ10 further demonstrated that the biosynthesis pathway of 4-AAQB from A. cinnamomea is closely related to CoQ.

4. Conclusion

This study compared the structures of 4-AAQB and CoQ and proposed that the biosynthesis pathway of 4-AAQB is similar and closely related to the biosynthesis of CoQ. The proposed biosynthesis pathway of 4-AAQB is given in Fig. 10. We have demonstrated that 4-HBA is a precursor of 4-AAQB and that it contributes to the formation ofthe benzoquinone ring of 4-AAQB. Since adding of both 4-HBA and oleic acid significantly increased the production of 4- AAQB, we suspect that the synthesis of the isoprenoid chain might also occur via the mevalonate pathway, as in the case of CoQ, and that oleic acid is not only a material used in the isoprenoid chain. It may also be a precursor of the gamma-lactone ring of 4-AAQB. Furthermore, the adding of coenzyme Q0 to the fermentation broth was found to be the most effective way to increase the production of 4-AAQB. (Although the biosynthesis pathway of coenzyme Q0 is still unclear, we suspect that coenzyme Q0 forms CoQ, after which CoQ is converted to 4-AAQB via unknown steps.) The biosynthesis of coenzyme Q0 is from polyketide pathway, and it can be converted to 4-AAQB via unknown steps.