دانلود رایگان مقاله MWCNT مبتنی بر ماتریس به عنوان پلت فرم برای چسبندگی و رشد سلول

Abstract

In this study we examine multiwall carbon nanotube (MWCNT) based nanosystems i.e. aligned MWCNT matrices, as potential scaffolds for cell growth and tissue engineering. We present the effect of samples purification and surface modification on the viability, adhesiveness and morphology of human normal gingival fibroblasts (HGF-1) and human osteosarcoma (U2OS) cell lines. We employ spectroscopic and analytical techniques (Scanning Electron Microscopy, Thermal Gravimetric Analysis, Atomic Force Microscopy, Raman and Fourier Transform Infrared Absorption Spectroscopy and Confocal Microscopy) in order to characterize the obtained structures. Furthermore, we analyze the expression level of selected cell adhesion-related genes by the quantitative real-time (qRT-PCR) method and the cell viability for MWCNT powders. We show that the surface structure of MWCNTs carpets contributes to the adhesion of cells. Additionally, we report the increased expression level of integrin, talin and fibronectin, proteins which are involved in cell attachment. We speculate that carbon nanotube based materials can be consider as potential candidates for biomedical purposes and as a biocompatible scaffold for cell growth.

4. Conclusion

The structural analysis revealed that modified MWCNT matrices have better quality than as-prepared ones. The high roughness factor of side walls of MWCNT carpets enables the cell attachment. Moreover, to obtain high hydrophilic and biocompatible surfaces, MWCNT carpets were defected in a controllable manner. The existence of carboxyl and aldehyde groups resulted in evident attachment of HGF-1 and U2OS cells for purified and oxidized samples. The F-actin cytoskeleton organization indicated cell growth in different directions on the matrix top surfaces. Additionally, the proper cell adhesion is confirmed by the increase of expression level of selected adhesion related genes (talin, integrin and fibronectin for U2OS). Rapid decrease of viability for U2OS and fibroblast cells was not observed. Our research showed that MWCNT based carpets are biocompatible with human bone osteosarcoma cells and gingival human fibroblasts, clearly showing that these materials may be used as matrices for future potential tissue engineering applications.