دانلود رایگان مقاله جداسازی، خالص سازی و فعالیت ایمنی پلی ساکارید جینسنگ آمریکایی

Abstract

In this study, crude American ginseng polysaccharide (AGPS) was extracted with hot water and preliminarily purified by using resin S-8 and Polyamide columns. Then, it was further purified and separated by DEAE-Sepharose CL-6B and Sepharose CL-6B chromatography, respectively. Five main fractions were obtained, named WPS-1, WPS-2, SPS-1, SPS-2 and SPS-3. Their homogeneities and structural characteristics were elucidated based on UV–vis spectroscopy, High Performance Gel Filtration Chromatography (HPGFC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectrum (IR), and NMR Spectroscopy methods. Furthermore, the immunostimulatory effects of these fractions upon splenic lymphocyte proliferation, macrophage phagocytosis and nitric oxide (NO) production, were investigated in vitro. The results indicated that their stimulations could be ordered as SPS-3 > SPS-1 > CPS (crude polysaccharides) > WPS-1 > WPS-2 > SPS-2. Among them, SPS-3 showed more potent immunomodulatory activity and could be explored as a potential immunopotentiating agent for use in functional food or medicine.

5. Conclusion

In summary, the present study is the first to demonstrate the purification of different polysaccharide fractions from American ginseng and to characterize some of their biological activities. The potency of their immunostimulatory effects can be ordered SPS- 3 > SPS-1 > CPS >WPS-1 >WPS-2 > SPS-2. These results indicated that fraction SPS-3 has high-efficiency therapeutic immunostimulatory properties and can be utilized as an immunoadjuvant or a functional food. These findings provide a basis for further investigation of the high-efficiency and specific immunobioactive constituents of the AGPS. However, the repeating units, sugar sequence, glycosidic linkage and the degree of branching of the potent component need further research. The details on the mechanisms and structure-activity relationship would be the subject of continuing study.