دانلود رایگان مقاله فعالیت ضد باکتری و cytocompatibility غشا پلی یورتان chitooligosaccharide

ABSTRACT

The aim of this study was to provide a convenient surface modification method for polyurethane (PU) membrane and evaluate its influence on hydrophilicity, antibacterial activity and cell functions, which are the most important factors for wound dressings. For this purpose, chitooligosaccharide (COS) was modified onto the surface of PU membrane based on the self-polymerization of dopamine (DOPA). Surface composition, morphology, hydrophilicity and surface energy of the original and modified PU membranes were characterized. Surface roughness and hydrophilicity of the PU membrane were obviously increased by modified with polydopamine (PDOPA) and COS. Antibacterial experiment against Escherichia coli and Staphylococcus aureus indicated that antibacterial activity of PU membrane increased only slightly by modified with PDOPA, but increased significantly by further modified with COS. Cells culture results revealed that COS-functionalized PU membrane is more beneficial to the adhesion and proliferation of NIH-3T3 cells compared to the original and PDOPA-modified PU membranes.

4. Conclusions

In this work, a simple and effective surface modification approach for PU membrane was conducted. TGA and XPS measurements revealed that PDOPA and COS have been successively immobilized on the surface of PU membrane. The rougher surface created by PDOPA and COS enabled the PU membrane to possess higher surface energy and better hydrophilicity, which were more in favor of the attachment and proliferation of NIH-3T3 cells. In addition, the antibacterial activity of PU membrane slightly increased by modified with PDOPA, but significantly increased by modified with COS. As a consequence, the D-PU and C-D-PU membranes, especially the latter, are not only in favor of preserving moisture and accelerating wound healing but also can increase the antibacterial activity. Thus the resulting C-D-PU membrane can be expected to have potential applications as a wound dressing material.