دانلود رایگان مقاله بررسی پلاسما ناشی از عوامل الکتریکی و شیمیایی

عنوان فارسی
بررسی پلاسما ناشی از عوامل الکتریکی و شیمیایی و پردازش سهم آنها برای انتقال ژن پلاسما
عنوان انگلیسی
Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection
صفحات مقاله فارسی
0
صفحات مقاله انگلیسی
21
سال انتشار
2016
نشریه
الزویر - Elsevier
فرمت مقاله انگلیسی
PDF
کد محصول
E354
رشته های مرتبط با این مقاله
زیست شناسی
گرایش های مرتبط با این مقاله
علوم سلولی مولکولی و بیوشیمی
مجله
ارشیو بیوشیمی و بیوفیزیک
دانشگاه
گروه مهندسی برق و الکترونیک، دانشگاه اهیمه، توکیو، ژاپن
کلمات کلیدی
انتقال ژن پلاسما، نفوذ غشاء، عملکرد غشاء، اندوسیتوز، اثر هم افزایی، پزشکی پلاسما
۰.۰ (بدون امتیاز)
امتیاز دهید
چکیده

Abstract


This study has been done to know what kind of factors in plasmas and processes on cells induce plasma gene transfection. We evaluated the contribution weight of three groups of the effects and processes, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the laser produced plasma (LPP) was employed to estimate the contribution of the chemical factors. Second, liposomes were fabricated and employed to evaluate the effects of plasma irradiation on membrane under the condition without biochemical reaction. Third, the clathrin-dependent endocytosis, one of the biochemical processes was suppressed. It becomes clear that chemical factors (radicals and reactive oxygen/nitrogen species) do not work by itself alone and electrical factors (electrical current, charge and field) are essential to plasma gene transfection. It turned out the clathrin-dependent endocytosis is the process of the transfection against the 60% in all the transfected cells. The endocytosis and electrical poration are dominant in plasma gene transfection, and neither permeation through ion channels nor chemical poration is dominant processes. The simultaneous achievement of high transfection efficiency and high cell survivability is attributed to the optimization of the contribution weight among three groups of processes by controlling the weight of electrical and chemical factors.

نتیجه گیری

5. Conclusion


We evaluated the contribution ratio of three groups of the effects and processes shown in Fig. 1, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the LPP was employed to estimate the contribution of the chemical factors. Second, liposomes were fabricated and employed to evaluate the effects of plasma irradiation on membrane under the condition without biochemical reaction. Third, the clathrin-dependent endocytosis, one of the biomedical processes was suppressed. It becomes clear that chemical factors do not work by itself alone and electrical factors are essential to plasma gene transfection. It turned out that in the plasma gene transfection the clathrin-dependent endocytosis is the process of the transfection against the 60% in all the transfected cells. The endocytosis and electrical poration are dominant in plasma gene transfection, and neither permeation through ion channels nor chemical poration is dominant processes.


بدون دیدگاه