دانلود رایگان مقاله مطالعات هموسازگاری و ضد میکروبی برای سنتز و اصلاح صمغ عربی میکروگلاس

ABSTRACT

Gum Arabic (GA) microgels were successfully prepared via reverse micellization method with high yield (78.5 ± 5.0%) in 5–100 m size range using divinyl sulfone (DVS) as a crosslinker. The GA microgels were degraded hydrolytically 22.8 ± 3.5% at pH 1 in 20 days, whereas no degradation was observed at pH 7.4 and pH 9 at 37 ◦C. By using diethylenetriamine (DETA), and taurine (TA) as chemical modifying agents, GA microgels were chemically modified as GA-DETA and GA-TA, and the zeta potential values of 5.2 ± 4.1 and −24.8 ± 1.3 mV were measured, respectively in comparison to −27.3 ± 4.2 mV for GA. Moreover, blood compatibility of GA, GA-TA, and GA-DETA microgels was tested via in vitro protein adsorption, % hemolysis ratio, and blood clotting index. All the microgels were hemocompatible with% hemolysis ratio between 0.23 to 2.05, and the GA microgels were found to be highly compatible with a blood clotting index of 81 ± 40. The biocompatibility of GA, GA-DETA and GA-Taurine microgels against L929 fibroblast cells also revealed 84.4, 89.1, and 67.0% cell viability, respectively, at 25.0 g/mL concentration, suggesting great potential in vivo biomedical applications up to this concentration. In addition, 5 and 10 mg/mL minimum inhibition concentrations of protonated GA-DETA microgels (GA-DETA-HCl) were determined against E. coli and S. aureus, respectively.

4. Conclusions

This preliminary study shows a one-pot route for the synthesis of GA microgels via water-in-oil micro-emulsion polymerization technique with a high yield of 78.5 ± 5.0%. The as prepared GA microgels have superficial hydroxyl groups which gives −27.31 ± 4.2 mV overall surface charge. GA microgels were further modified with DETA and TA to have different functional groups, and it was shown that GA microgels can render a surface charge up to +27 ± 15 mV for additional characteristics such as bactericidal properties. Similarly, GA microgels show 22.8 ± 3.5 biodegradability property at stomach condition (pH 1) in 20 days. Moreover, GA, GA-DETA and GA-TA microgels exhibit high hemocompatibility with high protein adsorption capacity, low hemolysis effects for blood and possess low apoptotic and neurotic indices up to 50 g/mL. Furthermore, the protonated DETA modified GA show a pronounced killing effect against gram negative and gram positive bacteria species. Therefore, bare and modified GA microgels can be used as bio-devices for potential biological applications with their tunable chemical and physical properties with various size and surface charges via chemical modification prepared as reported here can be considered as multipurpose material with biological origin and great potential in biomedical field.