- مبلغ: ۸۶,۰۰۰ تومان
- مبلغ: ۹۱,۰۰۰ تومان
Super porous gum Arabic (GA) cryogels were synthesized by crosslinking of natural GA with divinyl sulfone at cryogenic conditions, −20°C for potential biomedical applications. Humic acid (HA) nanoparticles were also prepared by using degradable and biocompatible crosslinkers such as trimethylolpropane triglycidyl ether, poly(ethylene glycol) diglycidyl ether, and trisodium trimetaphosphate in a single step and then entrapped within GA cryogel network as GA/HA particle cryogel. Furthermore, GA/HA cryogel was used as a template for Ag, Cu, and Fe nanoparticle preparation, and their antimicrobial properties were tested against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis strains. The minimum inhibition concentration values of Ag and Cu nanoparticle‐loaded GA/HA cryogel composites were determined as 10 mg mL−1 . Furthermore, the blood compatibility tests such as hemolysis and blood clotting indexes were determined for GA cryogels and found to be more compatible with 0.08 ± 0.01% hemolysis and 89.4 ± 6.1 blood clotting values, whereas the hemolysis of the Ag, Cu, and Fe nanoparticle‐loaded GA/HA Ag, Cu, and Fe metal nanoparticle cryogel composites decreased in the order of Fe > Cu > Ag nanoparticles.
In this study, HA particles from natural source were successfully synthesized by using 3 different biocompatible crosslinkers, TMP, PEGGE, and STMP, and included into super porous GA cryogel network to prepare GA/HA cryogels for potential biomedical applications. It was also demonstrated here that GA/HA cryogels were used as a template for in situ Ag, Cu, and Fe NP preparations. It was found that Ag and Cu NP‐loaded GA/HA‐M cryogel composites are effective antimicrobial materials against Gram negative and Gram positive bacteria strains such as E. coli, S. aureus, and B. subtilis, whereas blood compatibility revealed that the existence of MNP in the cryogel network decreased the biocompatibility of the GA/HA‐M cryogel composites. Furthermore, the results for in vitro blood compatibility suggested that GA cryogels are blood compatible with 0.08 ± 0.01% hemolysis and 89.4 ± 6.1 blood clotting values. Therefore, the prepared microgel and microgel‐embedded cryogel networks that were prepared from natural sources such as HA and GA are versatile materials and have great potential to be used in many biomedical applications.