دانلود رایگان مقاله انگلیسی فعالیت های آنتی اکسیدانی آزمایشگاهی عصاره ریشه مارچوبه racemosus Linn - الزویر 2018

abstract

Objective: The purpose of the study is to investigate potential of antioxidant property of ethanolic root extract of Asparagus racemosus Linn (EEAR). Methods: In vitro evaluation antioxidant property of EEAR was done using various methods like DPPH scavenging activity, hydroxyl radical scavenging activity, and nitric oxide scavenging activity. HPTLC fingerprint analysis was performed for qualitative determination of possible number of components from the ethanolic extract. Acute toxicity study was performed in Wistar rat and an OECD guideline 423 was followed. Results: The yield value was found 0.96% from EEAR. A concentration of 468.57 ± 3.002 mg/ml of probable antioxidant material from EEAR was required to scavenge 50% of DPPH. The IC50 value of EEAR were found to be 508.17 ± 7.37 mg and 416.57 ± 5.08 mg when determined by hydroxyl radical and nitric oxide scavenging assay respectively. The reducing powers of EEAR was 0.295 ± 0.0037 at 125 mg/ml and increased to 0.934 ± 0.0005 at 500 mg/ml. HPTLC fingerprint data supports several basic informations like isolation, purification, quality evaluation and standardization. No sign of toxicity was observed after treated with 2000 mg/kg of EEAR. Conclusion: The obtained data highlight the potential role of EEAR as a source of natural antioxidants.

5. Conclusion

EEAR showed relevant antioxidant property. This study provides experimental support for the traditional medicinal plants. To ensure therapeutic efficacy and quality control of the drug along with its identification, this HPTLC data will serve as reference standard for scientists engaged. So along with antioxidant property of this plant, HPTLC fingerprint data of root extract of EEAR can be used as diagnostic tool for the correct identification of the plant and also useful to estimate genetic variability in their population. Based on the toxicity study, EEAR was found to be non-toxic at a dose of 2000 mg/kg. Still molecular studies of the above mentioned plant may open up new hope in drug discovery and research.